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- St. Ann's Church Catholic (Latin rite) · Church
- St. Augustine's Church Catholic (Latin rite) · Church
- Ancaster Canadian Reformed Church Reformed and Presbyterian · Church · 575 Shaver Road
- Turning Point Church Pentecostal and Charismatic · Church · 1151 Garner Road West
- Ancaster Christian Reformed Church Reformed and Presbyterian · Church
- Bethel Community Gospel Centre House of prayer · 4457 Bethel Church Road
- Bethesda United Church House of prayer · 542 Garner Road West
- Bowman United Church Church · 880 Garner Road East
- Calvary Christian Reformed Church Reformed and Presbyterian · Church
- Christan Life Assembly Church
- Christ Church Flamborough Anglican · Church · 92 Highway 8
- Heritage Fellowship Baptist Church Baptist · House of prayer · 557 Garner Road West
- Kingdom Hall of Jehovah's Witnesses Other restorationist · House of prayer
- Knox Presbyterian Church Reformed and Presbyterian · Church
- Meadowlands Fellowship Christian Reformed Church Reformed and Presbyterian · Church
- Mount Mary Immaculate Retreat Centre Catholic (Latin rite) · Church
- St. Andrew's Presbyterian Church Reformed and Presbyterian · Church
- St. James Anglican Church Anglican · Church
- St. John's Anglican Church Anglican · House of prayer
- Unity Church House of prayer · Unity Side Road
- West Flamborough Presbyterian Church Reformed and Presbyterian · Church
- White Brick Church Church